Zymodemes of Trypanosoma congolense and a preliminary assessment of their epidemiological significance
Throughout this century, considerable confusion has arisen over the specific status of members of the subgenus Nannomonas. At the present time, only two species are generally recognized, T.(N.) congolense and T.(N.) simiae. However, many differences still exist in morphological and behavioural characteristics of T.congolense stocks, which led in the past, to numerous designations of species, subspecies and varieties. In an attempt to clarify the problems, 78 stocks of T.congolense were characterized intrinsically by enzyme polymorphism. Conditions devised for thin-layer starch-gel electrophoresis of the isoenzymes of trypanosomes from other subgenera were modified for 14 enzymes: L-threonine 3-dehydrogenase (TDH), purine nucleoside hydrolase (NH), pyruvate kinase (PK), glyceraldehyde phosphate dehydrogenase (GAPDH), aspartate aminotransferase (ASAT), phoepho-glucomutase (PGM), alanine aminotransferase (ALAT), malate dehydro-genase (decarboxylating NADP) (ME), malate dehydrogenase (MDH), glucose phosphate isomerase (GPI) and four peptidases using either L-leucylglycylglycine (PEP 1) or L-leucyl-L-alanine (PEP 2) as substrate. Eight of these enzymes readily distinguished T.congolense from representatives of
Young, C. J.
Archaeology
Zymodemes of Trypanosoma congolense and a preliminary assessment of their epidemiological significance. Throughout this century, considerable confusion has arisen over the specific status of members of the subgenus Nannomonas. At the present time, only two species are generally recognized, T.(N.) congolense and T.(N.) simiae. However, many differences still exist in morphological and behavioural characteristics of T.congolense stocks, which led in the past, to numerous designations of species, subspecies and varieties. In an attempt to clarify the problems, 78 stocks of T.congolense were characterized intrinsically by enzyme polymorphism. Conditions devised for thin-layer starch-gel electrophoresis of the isoenzymes of trypanosomes from other subgenera were modified for 14 enzymes: L-threonine 3-dehydrogenase (TDH), purine nucleoside hydrolase (NH), pyruvate kinase (PK), glyceraldehyde phosphate dehydrogenase (GAPDH), aspartate aminotransferase (ASAT), phoepho-glucomutase (PGM), alanine aminotransferase (ALAT), malate dehydro-genase (decarboxylating NADP) (ME), malate dehydrogenase (MDH), glucose phosphate isomerase (GPI) and four peptidases using either L-leucylglycylglycine (PEP 1) or L-leucyl-L-alanine (PEP 2) as substrate. Eight of these enzymes readily distinguished T.congolense from representatives of
preliminary assessment epidemiological significance throughout_century considerable confusion arisen specific status member present time two_specie generally recognize however many difference still exist morphological behavioural characteristic stock lead past numerous designation specie variety attempt_clarify problem stock characterize intrinsically enzyme conditions devise thin layer starch modify enzyme phosphate phosphate use either substrate enzyme readily distinguish representative