Untangled : species identification of silks by protein mass spectrometry
Historical records and archaeological findings suggest the commercial use of wild silks in the ancient Middle East and Europe before the dissemination of Chinese silk. However, scientific analysis of archaeological textiles is often hindered by their physical durability and the analytical capacity of conventional microscopic methods, and thus limitations of accurate identification have resulted in many controversies. This research aims to promote the comprehensive understanding of silk, by providing literature reviews in the entomological, historical and physico-chemical aspects of the material. On this foundation, the experimental works were designed to establish the potential methods of protein mass spectrometry of silk fibroins. The objectives of the experiments were to distinguish and to identify the different species of silk. Firstly, a study on the solubilisation of silk fibroins using domesticated and wild silk species was implemented. Then the development of analytical protocols for the protein mass spectrometry of silk fibroins followed, employing peptide sequencing using tandem mass spectrometry as the primary tool of analysis. The resulting analytical protocol enabled the identification and distinction
Lee, Boyoung
Untangled : species identification of silks by protein mass spectrometry. Historical records and archaeological findings suggest the commercial use of wild silks in the ancient Middle East and Europe before the dissemination of Chinese silk. However, scientific analysis of archaeological textiles is often hindered by their physical durability and the analytical capacity of conventional microscopic methods, and thus limitations of accurate identification have resulted in many controversies. This research aims to promote the comprehensive understanding of silk, by providing literature reviews in the entomological, historical and physico-chemical aspects of the material. On this foundation, the experimental works were designed to establish the potential methods of protein mass spectrometry of silk fibroins. The objectives of the experiments were to distinguish and to identify the different species of silk. Firstly, a study on the solubilisation of silk fibroins using domesticated and wild silk species was implemented. Then the development of analytical protocols for the protein mass spectrometry of silk fibroins followed, employing peptide sequencing using tandem mass spectrometry as the primary tool of analysis. The resulting analytical protocol enabled the identification and distinction
specie_identification silk protein mass_spectrometry historical_record archaeological_finding suggest commercial use wild silk ancient middle east europe dissemination chinese silk however scientific_analysis archaeological textile often hinder physical durability analytical capacity conventional microscopic method thus limitation accurate identification result many controversy aim_promote comprehensive silk provide literature_review historical chemical aspect material foundation experimental_work design establish potential method protein mass_spectrometry silk objective distinguish identify different specie silk firstly study silk use domesticated wild silk specie implement development analytical protocol protein mass_spectrometry silk follow employ sequence use tandem mass_spectrometry primary tool analysis result analytical protocol enable identification distinction